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【产品介绍】Glyceraldehyde-3-phosphate dehydrogenase
(GAPDH) was considered a classical glycolytic
protein involved exclusively in cytosolic energy
production. However, recent evidence suggests that it is
a multifunctional protein displaying diverse
activities distinct from its conventional metabolic
role. These new roles f o r GAPDH may be dependent on
its subcellular localization, oligomeric state o r on
the proliferative state of the cell. Results suggest
that human cells contain significant intracellular levels
of enzymatically inactive GAPDH which is age-
independent. The possibility is considered that
the functional diversity of GAPDH may be mediated either
by posttranslational alteration o r by
subcellular protein:protein a n d / o r protein:nucleic
acid interactions (1). GAPDH mRNA levels, protein,
a n d enzymatic activity have been shown to
increase increase in 3T3 cells after exposure
to physiological concentrations of insulin (2).Recommended Applications
WB, IHC, ICC, IP
Applications and Recommended Dilution Factors
WB: 1:1000
IHC: 1:100
ICC: 1:50
IP: 1:40
Species Reactivity *
Human, Mouse, Rat
* *Cross reactivity determined by western blot only.
Products Data A. Western blot analysis on (A) NIH/3T3 or (B) HeLa
cell lysate using anti-GAPDH RabMAb
(cat. # 2251-1), dilution 1:2000. B. Immunohistochemical analysis of paraffin-embedded
human colon adenocarcinoma using anti-GAPDH RabMAb
(cat. #2251-1).
Specificity
A synthetic peptide corresponding to residues in human
GAPDH was used as an immunogen. |
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